Filtration of Cell Culture Supernatants

摘要

The invention relates to a method for separating off a liquid supernatant from cells, comprising the steps: providing a mixture of the cells with a liquid, charging a first filter housing with the mixture, wherein in the filter housing a filter having a pore size of between 4 μm and 50 μm is provided on a flat base surface pierced in a sieve manner and the walls of the filter housing are connected so as to seal with the flat base surface that is pierced in a sieve manner, applying a differential pressure of at least 0.5 bar on the mixture, as a result of which the liquid portion of the mixture is forced through the filter and a filter cake containing cells remains in the filter housing, and removal of the filtered liquid.

主权项

1. A method for separating a fluid supernatant from cells, comprising the following steps:
a) providing a mixture of the cells with a fluid, b) charging a filter housing with the mixture, wherein in the filter housing a first filter having a pore size in the range 4 μm to 50 μm is provided on a flat base surface and the walls of the filter housing are connected so as to seal with the flat base surface which is perforated in the manner of a sieve, c) applying a differential pressure of at least 0.5 bar to the mixture, as a result of which the fluid portion of the mixture is forced through the filter and a filter cake containing cells remains in the filter housing, d) removal of the filtered fluid.