METHOD FOR PREPARING TRANSFERRABLE NANOSCALE TRANSFERRABLE MEMBRANE AND USE THEREOF

摘要

Disclosed is a method for preparing a transferable membrane having a nanometer scale dimension in thickness and pore size by non-solvent vapor-induced phase separation process, comprising spin-casting a polymer solution in a closed humid chamber and controlling the relative humidity (RH) of the chamber using at least one supersaturated salts solution whereby the density of the pores are controlled. Also provided is a TNT membrane prepared by the present method and its use. The present membrane can be advantageously used as co-culture platform facilitating versatile and controllable cell co-culture assays and further allowing the quantitative analysis of paracrine communications between cells for example between cancer cells and different types of stromal cells by providing an in vivo-like environment, which can offer more in-vivo-like results to identify key signaling molecules for therapeutic targets of a disease.

主权项

1. A method for preparing a transferable membrane having a nanometer scale dimension in thickness and pore size by non-solvent vapor-induced phase separation process, the method comprising spin-casting a polymer solution in a closed humid chamber and controlling the relative humidity (RH) of the chamber using at least one supersaturated salts solution whereby the size of the pores is controlled.