发明名称 |
SPATIAL MOLECULAR BARCODING OF IN SITU NUCLEIC ACIDS |
摘要 |
This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described. |
申请公布号 |
US2015148239(A1) |
申请公布日期 |
2015.05.28 |
申请号 |
US201414493127 |
申请日期 |
2014.09.22 |
申请人 |
Agilent Technologies, Inc. |
发明人 |
Peter Brian Jon;Ach Robert A.;Scheffer-Wong Alicia;Caffaro Carolina |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method of sample analysis, comprising:
(a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the location of the oligonucleotide on the solid support; (c) hybridizing the nucleic acid tags, or a copy of the same, with said oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in said duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. |
地址 |
Loveland CO US |