摘要 |
The invention relates to a method for determining interaction kinetics for an analyte. The method comprises first contacting a solution containing the analyte with immobilized ligand, or analogue thereof, immobilized on an optical sensor surface; monitoring the binding of the analyte to the immobilized ligand or analogue, wherein the binding is measured as a resulting change in a property of the surface; and automatically determining the interaction kinetics, which determining step includes first defining parts of the dissociation phase that contains kinetic information for fitting. The invention further relates to an analytical system for studying molecular interactions, which system is capable of performing the novel method, as well as a computer program product for performing the steps of the method. |