3D Imaging of Live Cells with Ultraviolet Radiation

摘要

A method for 3D imaging of cells in an optical tomography system (11) includes moving a biological object (1) relatively to a microscope objective (18) to present varying angles of view. The biological object (1) is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object (1) and the microscope objective (18) is sensed with a camera (48) from a plurality of differing view angles. A plurality of pseudoprojections of the biological object (1) from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.