METHOD TO OBTAIN OSTEOBLAST OR CELL CAPABLE OF DIFFERENTIATING INTO OSTEOBLAST, CAPABLE OF ANALYZING CELL SURFACE MARKER BY FLOW CYTOMETRY

摘要

PROBLEM TO BE SOLVED: To provide a method to obtain osteoblasts or cells capable of differentiating into osteoblasts, capable of analyzing a cell surface marker by flow cytometry.SOLUTION: A method for removing calcification material from osteoblasts or cells capable of differentiating into osteoblasts, includes a step in which two kinds of density gradient medium liquids with different concentrations are superposed, and further the osteoblasts or cells capable of differentiating into osteoblasts are superposed on an upper layer thereof for centrifugation.