ANTIBODY AGAINST TRANSPORTER AND USE THEREOF

摘要

The present invention provides a prophylactic or therapeutic agent for various malignant tumors, including currently intractable solid tumors, which contains a novel antibody having the ability to bind to human LAT1/CD98 and inducing antibody-dependent cellular cytotoxicity specifically against cancer cells as an active ingredient.

主权项

1. An antibody or an antigen-binding fragment thereof, each specifically binding to the extracellular region of the LAT1/CD98 complex in LAT1/CD98-expressing cells, which is:
(1) an antibody or an antigen-binding fragment thereof, which comprises, as light chain complementarity determining region 1 (CDRL1), light chain complementarity determining region 2 (CDRL2) and light chain complementarity determining region 3 (CDRL3), the amino acid sequences of SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 9, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 9, respectively, and which comprises, as heavy chain complementarity determining region 1 (CDRH1), heavy chain complementarity determining region 2 (CDRH2) and heavy chain complementarity determining region 3 (CDRH3), the amino acid sequences of SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, respectively; (2) an antibody or an antigen-binding fragment thereof, which comprises, as CDRL1, CDRL2 and CDRL3, the amino acid sequences of SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15, respectively, and which comprises, as CDRH1, CDRH2 and CDRH3, the amino acid sequences of SEQ ID NO: 16, SEQ ID NO: 17 and SEQ ID NO: 18, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 16, SEQ ID NO: 17 and SEQ ID NO: 18, respectively; (3) an antibody or an antigen-binding fragment thereof, which comprises, as CDRL1, CDRL2 and CDRL3, the amino acid sequences of SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21, respectively, and which comprises, as CDRH1, CDRH2 and CDRH3, the amino acid sequences of SEQ ID NO: 22, SEQ ID NO: 23 and SEQ ID NO: 24, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 22, SEQ ID NO: 23 and SEQ ID NO: 24, respectively; or (4) an antibody or an antigen-binding fragment thereof, which comprises, as CDRL1, CDRL2 and CDRL3, the amino acid sequences of SEQ ID NO: 25, SEQ ID NO: 26 and SEQ ID NO: 27, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 25, SEQ ID NO: 26 and SEQ ID NO: 27, respectively, and which comprises, as CDRH1, CDRH2 and CDRH3, the amino acid sequences of SEQ ID NO: 28, SEQ ID NO: 29 and SEQ ID NO: 30, respectively, or amino acid sequences modified to have mutation of one or several amino acid residues in the amino acid sequences of SEQ ID NO: 28, SEQ ID NO: 29 and SEQ ID NO: 30, respectively.