HIGH-THROUGHPUT IMAGE-BASED CHEMICAL SCREENING IN ZEBRAFISH BLASTOMERE CELL CULTURE

摘要

Disclosed are methods of inducing differentiation of stem into myogenic cells without gene manipulation and for inducing proliferation of satellite cells. The cells can be used as a source of cells for transplantation in a subject in need thereof. Also disclosed is a screening assay for screening test compounds using blastomere cultures.

主权项

1. A method of inducing differentiation of stem cell to myogenic cell, the method comprising culturing a stem cell with at least two of: (i) a GSK3 pathway inhibitor; (ii) a compound that increases intracellular levels of 3′,5′-cyclic adenosine monophosphate (cAMP); and (iii) a FGF pathway activator.