METHOD FOR MANUFACTURING TELENCEPHALON OR PROGENITOR TISSUE THEREOF

摘要

The present invention provides a method for inducing more mature telencephalon or progenitor tissue thereof in vitro from pluripotent mammalian stem cells, the method being characterized: in that telencephalon marker-positive aggregates are obtained by suspension culturing of pluripotent stem cell aggregates in the presence of Wnt signal inhibitor and TGFß signal inhibitor; and in further suspension culturing of said telencephalon marker-positive aggregates under high oxygen partial pressure conditions. In one embodiment, the suspension culturing under high oxygen partial pressure conditions is performed in the presence of Wnt signal enhancer and a substance that activates a bone morphogenetic factor signal transduction pathway.