PROGRAMMABLE ENZYMES FOR ISOLATION OF SPECIFIC DNA FRAGMENTS

摘要

Selective simultaneous in vitro isolation of multiple double-stranded DNA (dsDNA) fragments of interest from complex mixtures of dsDNA fragments using catalytically inactive programmable sequence specific nucleases. In vitro assembled ternary ribonucleoprotein complexes Cas9/crRNA/tracRNA recognize and bind to dsDNA fragments that contain nucleotide sequences complementary to guiding crRNA sequences forming dsDNA/Cas9/crRNA/tracRNA complexes.