摘要 |
<p>A method for quantitation of one or more biomolecules in a sample, the method comprising the steps of providing a sample holder comprising a porous membrane which comprises a hydrophilic region surrounded by a hydrophobic region for sample containment, contacting the hydrophilic region of the membrane with a sample volume, drying the sample volume on the membrane, and exposing the sample volume on the membrane to an infrared beam comprising a wavelength in the spectral range of 4000-400 cm -1 or any portion of the spectral range of 4000-400 cm -1 , thereby to obtain an infrared absorption spectrum and wherein one or more absorption peak areas in the infrared absorption spectrum correlates with the quantity of the one or more biomolecules in the sample.</p> |