| 摘要 |
FIELD: medicine.SUBSTANCE: invention refers to biotechnology, namely to a method for identifying tuberculosis mycobacteria of cluster Beijing B0/W148. A polymerase chain reaction is conducted for amplification of M. tuberculosis DNA fragments with selected primers P1, P2 and P3, sequences of which are presented by SEQ ID NO 1, 2, 3, respectively in the amplification mode of 98°C - 10 minutes, 30 cycles: 98°C - 30 sec, 64°C - 30 sec, 72°C - 50 sec, 72°C - 10 min, 4°C. The amplification products are separated by electrophoresis in 2% agarose gel. If the fragment of 1,021 base pairs is produced, the strain of tuberculosis mycobacteria of cluster Beijing B0/W148 is detected.EFFECT: presented invention enables fast and high-specificity identification of tuberculosis mycobacteria of cluster Beijing B0/W148.3 dwg |
