Biomolecule fixing board and method of manufacturing the same

摘要

This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.

主权项

1. A biomolecule modifying substrate comprising:
a substrate; a first surface on the substrate, the first surface comprising a metal oxide; a second surface on the substrate, the second surface comprising a noble metal; a first linker molecule comprising a hydrocarbon chain and a functional group at one end or in a side chain of the hydrocarbon chain, the functional group capable of selectively binding to the first surface, the first linker molecule bound to the first surface via the functional group; a second linker molecule comprising biotin and a reactive group, the reactive group capable of binding to the hydrocarbon chain of the first linker molecule, the second linker molecule bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; a biomolecule comprising an introduced biotin; and an avidin, wherein the biotin contained in the second linker molecule is bound to the biotin introduced into the biomolecule via the avidin, and wherein the functional group of the first linker molecule capable of selectively binding to the first surface is a phosphate group.