Method of selecting stem cells having high chondrogenic differentiation capability

摘要

Thrombospondin 1 (TSP-1), TSP-2, interleukin 17B receptor (IL-17BR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) associated with stem cell activity and use thereof.

主权项

1. A method of selecting a stem cell capable of differentiating into a chondrocyte or inducing chondrocyte differentiation, the method comprising:
culturing a stem cell in a monolayer or pellet in a maintenance medium; measuring a concentration of TSP-2; and selecting and isolating a stem cell having a high chondrogenic differentiation capability from the culture medium if a) the concentration of TSP-2 in cell culture is larger than 72 pg/ml/1.0×105 cells when the stem cell is monolayer cultured for 1 day, or b) the concentration of TSP-2 in cell culture is larger than 550 pg/ml/1.0×105 cells when the stem cell is pellet cultured for 7 days, wherein the selected stem cell is capable of differentiating into a chondrocyte or inducing chondrocyte differentiation.