| 发明名称 |
Method for increasing protein thermal stability |
| 摘要 |
The invention provides a simple and effective method for increasing thermal stability of a wide range of proteins, comprising fusing a self-assembling amphipathic peptide to the C- or N-terminal of target proteins. The fusion protein can have a half life up to 26 times longer than that of the wild type protein. |
| 申请公布号 |
US9040274(B2) |
申请公布日期 |
2015.05.26 |
| 申请号 |
US201313870509 |
申请日期 |
2013.04.25 |
| 申请人 |
Jiangnan University |
发明人 |
Chen Jian;Du Guocheng;Lu Xinyao;Liu Song;Zhang Juan |
| 分类号 |
C12N9/96;C12N9/02;C12N9/26;C12N9/10 |
主分类号 |
C12N9/96 |
| 代理机构 |
|
代理人 |
Chen Lili |
| 主权项 |
1. A method for increasing thermal stability of a target protein, comprising fusing a self-assembling amphipathic peptide (SAP) having an amino acid sequence set forth in SEQ ID NO:5 to said target protein by recombinant techniques to make a Target Protein-SAP fusion protein, wherein said Target protein-SAP fusion protein has increased thermal stability. |
| 地址 |
Wuxi, JS CN |
