Detection of human T1R expressing cells

摘要

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

主权项

1. A method of identifying T1R1 expressing cells comprising detecting cells that express a T1R1 taste receptor, wherein such taste receptor comprises a taste receptor polypeptide that is at least 90% identical to the T1R1 polypeptide of SEQ ID NO:17.