发明名称 Arsenic assay using ELISA
摘要 The invention relates to a process involving analyzing the presence of arsenic in a sample, comprising the following steps: (a) contacting a sample suspected of containing arsenic on a solid support functionalized with a DNA fragment comprising an ArsR protein binding site, site on which an ArsR protein is attached, the presence arsenic causing separation of the ArsR protein from the DNA fragment;(b) incubation of the sample in contact with the said support;(c) elimination of ArsR protein separated from the DNA fragment by washing the support after the incubation carried out in step (b); and(d) analyzing by an ELISA technique to detect the presence or absence of ArsR protein remaining attached to the DNA fragment.
申请公布号 US9040240(B2) 申请公布日期 2015.05.26
申请号 US201214001648 申请日期 2012.02.23
申请人 UNIVERSITE DE LORRAINE 发明人 Poupin Pascal
分类号 C12Q1/68;G01N33/53;G01N33/84 主分类号 C12Q1/68
代理机构 Cantor Colburn LLP 代理人 Cantor Colburn LLP
主权项 1. A process for analyzing arsenic in a sample, comprising the following steps: (a) contacting a sample suspected of containing arsenic on a solid support functionalized with a DNA fragment comprising an ArsR protein binding site, site on which an ArsR protein is attached, the presence of arsenic causing separation of the ArsR protein from the DNA fragment; (b) incubation of the sample in contact with the said support; (c) elimination of ArsR protein separated from the DNA fragment by washing the support after the incubation carried out in step (b); and (d) analyzing by an ELISA technique to detect the presence or absence of ArsR protein remaining attached to the DNA fragment.
地址 FR