Fluorescent pH detector system and related methods

摘要

Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.

主权项

1. A method for measuring the pH of a sample, comprising:
(a) irradiating a fluorescent species immobilized on a substrate with excitation light emanating from a probe, wherein the fluorescent species immobilized on the substrate is in liquid communication with a sample, wherein the excitation light has a wavelength sufficient to effect fluorescent emission from the fluorescent species, wherein the fluorescent species exhibits a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, the ratio of the first and second emission intensities being dependent on pH, and wherein the fluorescent species immobilized on the substrate is prepared by covalently coupling a benzo[c]xanthene linker compound to the substrate, wherein the benzo[c]xanthene linker compound is its salts, active esters, acid/base forms, and tautomers; and (b) measuring the first and second emission intensities to determine the pH of the sample.