METHOD FOR PREPARING THE PLANT PRODUCING BISPECIFIC ANTIBODY

摘要

The present invention relates to a method for preparing plants capable of producing bispecific antibodies. More specifically, the method includes the steps of: (a) forming a single chain antibody by connecting the heavy chain variable region (VH) and the light chain variable region (VL) of an monoclonal antibody by a linker; (b) preparing a transgenic plant by introducing the genes of the single chain antibody formed in step (a); (c) forming another single chain antibody by connecting the heavy chain variable region (VH) and the light chain variable region (VL) of another monoclonal antibody by the linker; (d) preparing another transgenic plant by introducing the genes of the single chain antibody formed in step (c); and (e) breeding the transgenic plants prepared in the step (b) and step (d). The present invention also relates to an antibody preparation method including a step of obtaining proteins from the transgenic plants prepared by the previous preparation method. The present invention can mass-produce the bispecific antibodies by using the preparation method and mass-produce the low-cost monoclonal antibodies.