| 摘要 |
<p>The present invention provides a method of expressing at least one heterologous nucleic acid sequence in a cell the method comprising introducing at least one heterologous nucleic acid sequence into a cell by infecting said cell with a recombinant negative strand RNA virus vector comprising said at least one heterologous nucleic acid sequence wherein the recombinant negative strand RNA virus vector includes a viral genome coding for a mutated P protein which leads to a loss of the viral genome replication ability without a loss of the viral transcription ability and wherein said at least one heterologous nucleic acid sequence encodes a cellular reprogramming or programming factor or a therapeutic protein. In addition the present invention provides a cell or a population of cells prepared in vitro by said method as well as a pharmaceutical composition comprising said cell or population of cells.</p> |
