METHOD, KIT AND PREMIX FOR QUANTITATIVE AND DISCRIMINATIVE DETERMINATION OF NUCLEIC ACIDS, QDPCR; IN VITRO METHOD FOR THE QUANTITATIVE AND DISCRIMINATIVE DIAGNOSIS OF BIOLOGICAL ENTITIES

摘要

The present invention provides a method and a number of compounds for a kit for the quantitative, specific and discriminative determination of nucleic acids (qdPCR). The products and methods of the invention correspond to a development that allows a quantitative diagnostic of a gene locus to be obtained, together with a method for determining and discriminating between gene aleles and loci or even between orthologous genes from different biological entities present in samples or assays, simultaneously and in a single closed system.