发明名称 |
METHOD OF IMMOBILIZING A PROTEIN OR MOLECULE VIA A MUTANT DEHALOGENASE THAT IS BOUND TO AN IMMOBILIZED DEHALOGENASE SUBSTRATE AND LINKED DIRECTLY OR INDIRECTLY TO THE PROTEIN OR MOLECULE |
摘要 |
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. |
申请公布号 |
US2015140587(A1) |
申请公布日期 |
2015.05.21 |
申请号 |
US201414330910 |
申请日期 |
2014.07.14 |
申请人 |
Promega Corporation |
发明人 |
Darzins Aldis;Encell Lance;Johnson Tonny;Klaubert Dieter;Los Georgyi V.;McDougall Mark;Wood Keith V.;Wood Monika G.;Zimprich Chad |
分类号 |
C12N9/14;C12Q1/34;C12Q1/66;C12N9/02 |
主分类号 |
C12N9/14 |
代理机构 |
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代理人 |
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主权项 |
1. (canceled) |
地址 |
Madison WI US |