MEDICINAL COMPOSITION OF EXTRACT OF SEED OF EMBLICA OFFICINALIS AND METHOD OF PREPARING THE SAME

摘要

A composition having an extract of seed of Emblica officinalis. Methods of preparing extract of seed of Emblica officinalis. An amla seed blend composition having various ratios of extracts of seeds of Emblica officinalis. Nutraceutical or pharmaceutical methods for decreasing the total cholesterol, decreasing triglyceride, decreasing blood glucose level, enhancing HDL-C levels, increasing the HDL-C level to total cholesterol ratio, lowering LDL-C levels, decreasing the CRP level, decreasing the intima media thickening, reducing hair fall in mammals especially human beings. The extract of seed of Emblica officinalis, or the amla seed blend composition is more effective compared to extracts prepared from fruits of Emblica officinalis.

主权项

1. A method of preparing an amla seed blend, the method comprising blending a Product 1 and a Product 2 to obtain the amla seed blend, wherein a method of preparing the Product 1 comprises:
deseeding fresh fruits of Emblica officinalis to obtain seeds of Emblica officinalis; crushing the seeds of Emblica officinalis to obtain crushed seeds; extracting the crushed seeds with 95% methanol to obtain a residue and a supernatant; concentrating the supernatant to obtain a concentrated methanol extract; drying the concentrated methanol extract to obtain a powder of methanol extract of seeds of Emblica officinalis; dispersing the powder of methanol extract of seeds of Emblica officinalis in water to obtain a dispersion; extracting the dispersion with hexane to obtain a water phase and a hexane phase; concentrating the hexane phase to obtain a liquid form of a concentrated hexane extract; cooling the liquid form of the concentrated hexane extract to obtain precipitates or crystals and a liquid portion; separating the liquid portion from the precipitates or crystals to obtain a liquid Product 1; extracting the water phase with ethyl acetate to obtain an ethyl acetate phase; concentrating the ethyl acetate phase to obtain a concentrated ethyl acetate phase; drying the concentrated ethyl acetate phase to obtain a powder of an ethyl acetate extract; mixing the powder of ethyl acetate extract with water to obtain a liquid for chromatographic separation; loading the liquid for chromatographic separation onto an ion exchange column; eluting the ion exchange column with water to obtain a water fraction (Fraction 1); concentrating the Fraction 1 to obtain a concentrate of Fraction 1; drying the concentrate of Fraction 1 to obtain a powder of Fraction 1; eluting the ion exchange column with 50% methanol to obtain a Fraction 2; concentrating the Fraction 2 to obtain a concentrate of Fraction 2; drying the concentrate of Fraction 2 to obtain a powder of Fraction 2; eluting the ion exchange column with 80% methanol to obtain a Fraction 3; concentrating the Fraction 3 to obtain a concentrate of Fraction 3; drying the concentrate of Fraction 3 to obtain a powder of Fraction 3; combining Fraction 1, Fraction 2 and Fraction 3 to obtain Product 2; blending Product 2 and Product 1 in a ratio of Product 2 to Product 1, wherein the ratio ranges from about 1:60 to about 99:1, and whereby the amla seed blend is obtained.