| 摘要 |
The invention relates to an arrangement for light sheet microscopy. Such an arrangement comprises lighting optics with a lighting lens (5) for illuminating a sample (3) located on a sample carrier in a medium (2), by means of a lighting beam path with a light sheet, wherein the optical axis (6) of the lighting lens (5) and the light sheet lie in a plane which encloses with the normal of a planar reference face (4), with respect to which the sample carrier is aligned, a lighting angle (ß) which is different from zero. The arrangement furthermore comprises a detection optical unit comprising a detection lens (7) in a detection beam path, the optical axis (8) of which encloses a detection angle (δ) which is different from zero with the normal to the reference face. Finally, the arrangement also comprises a separating layer system with at least one layer composed of a predefined material of a predefined thickness, which separates the medium (2) from the lighting lens (5) and the detection lens (7), wherein the separating layer system is in contact with the medium (2), with a base surface (9) oriented parallel to the reference face (4), at least in the region which is accessible to the lighting lens (5) and the detection lens (7) for the purpose of illuminating or detecting. The arrangement also comprises a correction lens system with at least one detection correction lens for reducing such aberrations which arise owing to the oblique passage of light to be detected through boundary faces of the separating layer system. In such an arrangement, the at least one detection correction lens (9) is configured as a free-form lens and is arranged between the detection lens (7) and the separating layer system. Alternatively, it also forms the front lens of the detection lens (7). |
