METHOD FOR PRODUCING PANCREATIC HORMONE-PRODUCING CELL, PANCREATIC HORMONE-PRODUCING CELL, AND DIFFERENTIATION/INDUCTION PROMOTER

摘要

The method for producing a pancreatic hormone-producing cell according to the present invention is characterized in that a specific differentiation/induction promoter is added to a culture medium in the process of differentiating/inducing of a pluripotent stem cell or a pancreatic tissue stem/progenitor cell into the pancreatic hormone-producing cell. The differentiation/induction promoter to be used is: a polypeptide which comprises an amino acid sequence encoded by DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or a variant thereof; or a culture supernatant of a cell into which DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or DNA capable of hybridizing with DNA comprising a nucleotide sequence complementary to the aforementioned DNA under stringent conditions is integrated as a foreign gene.

主权项

1. A method of producing a pancreatic hormone-producing cell from a pluripotent stem cell or a pancreas tissue stem/precursor cell, the method comprising
adding at least one differentiation-inducing promoter selected from (1) to (3) to a culture medium in a differentiation-inducing process from the pluripotent stem cell or the pancreas tissue stem/precursor cell to the pancreatic hormone-producing cell: (1) a polypeptide consisting of an amino acid sequence encoded by DNA consisting of the base sequence set forth in SEQ ID NO. 1, (2) a polypeptide consisting of an amino acid sequence having one or several amino acid substitutions, deletions, and/or additions in the amino acid sequence encoded by DNA consisting of the base sequence set forth in SEQ ID NO. 1, and having a differentiation-inducing promotion effect into a pancreatic hormone-producing cell, (3) a culture supernatant of cells into which DNA consisting of the base sequence set forth in SEQ ID NO. 1 or DNA hybridizing with DNA consisting of a base sequence complementary with the DNA under stringent conditions is incorporated as a foreign gene.