TREATMENT OF LEAF TISSUE FOR THE RECOVERY OF INTERSTITIAL RECOMBINANT PROTEINS BY THE APPLICATION OF CELL WALL DEGRADING ENZYMES

摘要

The present disclosure relates to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue, and increasing the yield of recovery of an apoplast-targeted recombinant protein from a plant tissue.

主权项

1. A method for recovering apoplast-targeted recombinant protein from plant tissue, the method comprising:
a first step of providing plant tissue transformed to express an apoplast-targeted recombinant protein; a second step of contacting the plant tissue with a cell wall-degrading enzyme; a third step of extracting the apoplast-targeted recombinant protein from the plant tissue; a fourth step of incubating the plant tissue for an incubation period; and a fifth step of extracting the apoplast-targeted recombinant protein from the plant tissue,wherein the fifth step extracts the apoplast-targeted recombinant protein synthesized after the third step.