MICROFLUIDIC DEVICE FOR GENERATING NEURAL CELLS TO SIMULATE POST-STROKE CONDITIONS

摘要

This application provides devices for modeling ischemic stroke conditions. The devices can be used to culture neurons and to subject a first population of the neurons to low-oxygen conditions and a second population of neurons to normoxic conditions. The neurons are cultured on a porous barrier, and on the other side of the barrier run one or more fluid-filled channels. By flowing fluid with different oxygen levels through the channels, one can deliver desired oxygen concentrations to the cells nearest those channels.

主权项

1. A kit comprising:
a cellular chamber made of a material suitable for cell culture; a base comprising a channel, wherein the channel has an inlet and an outlet, and the channel is sized to be proximal to a first region of the cellular chamber and distal from a second region of the cellular chamber; a porous membrane suitable as a substrate for cell culture, wherein the porous membrane is sized to separate the channel from the cellular chamber; a plurality of electrodes configured to measure trans-endothelial electrical resistance; and a means for securing the microporous membrane between the chamber and the channel.