ASSAYS FOR DETECTING GLUCOSIDASE ACTIVITY

摘要

The present invention relates to a method of detecting α-(1→6)-glucosidase activity in a sample. The method is for example useful for determining the limit dextrinase activity in a sample. The method involves use of an oligosaccharide substrate of the formula X-(glucoside)n-*(glucoside)m-Z—Y, where X is a blocking group, -* is a α-(1→6)-glucosidic linkage and Y is a detectable label. Upon cleavage of the α-(1→6)-glucosidic linkage, the detectable label is released and thus the α-(1→6)-glucosidase activity can be determined. The invention also relates to the oligosaccharide substrate per se.

主权项

1. A method of detecting α-(1→6)-glucosidase activity in a sample, the method comprising the steps of
a. Providing a sample; b. Providing an oligosaccharide substrate of the formula
X-(glucoside)n-*(glucoside)m-Z—Ywherein X is a blocking group capable of inhibiting cleavage by an exo-glucosidase; andY is a detectable label; andZ is either S or O or N; and-* is an α-(1→6)-glucosidic linkage; andn and m individually are integers in the range of 1 to 6; c. Optionally, providing at least one exo-glucosidase; d. Incubating the sample with the oligosaccharide substrate and optionally with the exo-glucosidase; e. Determining the presence of free detectable label,
wherein the presence of free detectable label is indicative of α-(1→6)-glucosidase activity in said sample.