Methods and Compositions for Phototransfer

摘要

Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.

主权项

1. A method for transferring coding agents from a first surface to a second surface, comprising:
a) providing: i) a plurality of binding agents conjugated to a plurality of coding agents through a photocleavable linker, and ii) a source of electromagnetic radiation; b) contacting said binding agents with a first surface, said first surface comprising binding partners to said binding agents, under conditions such that at least a portion of said binding agents bind said binding partners; c) illuminating said binding agents with radiation from said radiation source under conditions such that at least a portion of said coding agents are photocleaved from said binding agents; and d) capturing at least a portion of said coding agents on a second surface.