EXPANDING CELLS IN A BIOREACTOR

摘要

Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

主权项

1. A method of loading cells in a cell expansion system, the method comprising:
circulating fluid at a first flow rate through a bioreactor of a cell expansion system, wherein the fluid comprises a plurality of cells; reducing circulation of fluid from the first flow rate; maintaining the bioreactor in a first orientation for a first predetermined period of time to allow at least a portion of the plurality of cells to settle; after the first predetermined period of time, rotating the bioreactor to a second orientation that is about 180 degrees from the first orientation; after the rotating, maintaining the bioreactor in the second orientation for a second predetermined period of time to allow at least a second portion of the plurality of cells to settle; and expanding at least the first and the second portion of cells in the bioreactor.