ASYNCHRONOUS MAGNETIC BEAD ROTATION (AMBR) MICROVISCOMETER FOR ANALYSIS OF ANALYTES

摘要

The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.

主权项

1. A method of detecting a change in state of an analyte in a sample comprising:
(a) contacting the sample with a magnetic particle; (b) measuring a first instantaneous non-linear rotation rate of the magnetic particle in the sample at a first time; and (c) measuring a second instantaneous non-linear rotation rate of the magnetic particle in the sample at a second time Y; wherein a difference between the first instantaneous rotation rate relative to the second instantaneous rotation rate indicates the change in state of the analyte in the sample.