METHOD FOR DETECTING INJURY TO THE BRAIN

摘要

Provided is a method for detecting injury to the brain comprising: a) determining the level of a tight junction (TJ) protein in exosomes isolated from a test sample from a subject, wherein the TJ protein is occludin, claudin-3, claudin-5, claudin-12, ZO-1, ZO-2, ZO-3, JAM-A, JAM-B or JAM-C, or any combination thereof; b) comparing the level of the TJ protein in the test sample to the level of the TJ protein in a control sample, wherein an elevated level of the TJ protein in the test sample relative to the level of the TJ protein in the control sample indicates that the subject has an injury to the brain.

主权项

1. A method for screening subjects for injury to the brain comprising:
a) determining the level of a tight junction (TJ) protein in exosomes from a test sample comprising blood, blood plasma, blood serum, cerebrospinal fluid or urine from a subject, wherein said TJ protein is occludin, claudin-3, claudin-5, claudin-12, ZO-1, ZO-2, ZO-3, JAM-A, JAM-B or JAM-C, or any combination thereof; and b) comparing the level of said TJ protein in said exosomes from said test sample to the level of the TJ protein in a control, wherein an elevated level of the TJ protein in said exosomes from said test sample relative to the level of the TJ protein in said control indicates that said subject has sustained an injury to the brain.