发明名称 |
3D CELL CULTURE AND EX VIVO DRUG TESTING METHODS |
摘要 |
Provided herein are methods for testing proliferative responses of a drug on patient-derived tumor cells; the method comprising obtaining cells from biopsy or tumor resection material; culturing the cells on a 3D extracellular matrix (ECM); treating the cells in ECM with a drug; subjecting the treated cells to high-content (HC) imaging; and evaluating the HC imaging of the treated cells; thereby testing the proliferative responses of the drug on the patient-derived tumor cells. In some embodiments, the methods disclosed herein comprise obtaining cells from biopsy or tumor resection material; xenografting the cells into an animal model (patient-derived xenograft; PDX) for tumor formation; and obtaining tumor cells from the animal. |
申请公布号 |
WO2015073724(A1) |
申请公布日期 |
2015.05.21 |
申请号 |
WO2014US65549 |
申请日期 |
2014.11.13 |
申请人 |
MOLECULAR RESPONSE, LLC |
发明人 |
NAIR, PRAVEEN;MIRSAIDI, CYRUS;BROUDY, TOMMY |
分类号 |
G01N33/50 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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