Methods and Compositions for Identifying and Validating Modulators of Cell Fate

摘要

The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.

主权项

1. A nucleic acid construct comprising:
(a) an open reading frame (ORF) encoding a reporter wherein the ORF is operably linked to a cell type related (CTR) promoter; and (b) a nucleic acid sequence encoding a target sequence RNA1 (“TSR1”).