METHOD FOR ANALYZING WATER TOXICITY

摘要

A method for analyzing water toxicity, the method including: exposure experiment, sample collection, transcriptome detection, metabolome detection, screening of differentially expressed genes, screening of differentially expressed metabolites, and identification of commonly changed biological pathways in both the transcriptome and the metabolome.

主权项

1. A method for analyzing water toxicity, the method comprising the following steps:
1) exposure experiment: selecting a water sample as an experimental group, using purified water as a blank control group, selecting model animals, administering the water sample to the model animals in the experimental group, and administering the purified water to the model animals in the blank control group; 2) sample collection: after a cycle of the exposure experiment, extracting total RNA samples of livers of the model animals; collecting blood samples, and centrifuging the blood samples to obtain serum samples; 3) transcriptome detection: mixing every three RNA samples to yield one sample, conducting transcriptome microarray detection; providing three gene chips for the experimental group and the blank control group, respectively; and conducting statistics analysis, whereby obtaining complete genome expression data of model animals; 4) metabolome detection: conducting nuclear magnetic resonance (NMR) detection on the serum sample of each model animal of the experimental group and the blank control group to yield NMR spectra of serum, whereby obtaining signal values of serum metabolites; 5) screening of differentially expressed genes: comparing gene expression signals of the experimental group with the gene expression signals of the blank control group according to detection results by the gene chips; screening genes having an expression multiple >2.0 and a false discovery rate (FDR)<0.1 as the differentially expressed genes; 6) screening of differentially expressed metabolites: performing segmental integral on the NMR spectra of serum, conducting partial least squares discriminant analysis (PLS-DA) on obtained integral data, and screening metabolites having variable influence on projection (VIP) >1.0 and FDR <0.01 as the differentially expressed metabolites; and 7) identification of biological pathways: conducting biological pathway analyses of the transcriptome and the metabolome, respectively, on the differentially expressed genes and differentially expressed metabolites; and identifying the biological pathways where both the transcriptome and the metabolome vary.