DEVICES AND METHODS FOR NUCLEIC ACID PREPARATION AND ANALYSIS

摘要

The invention provides in part a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA. The invention also provides in part structures and methods that allow polymers of any length, including nucleic acids, to be stretched into a long, linear conformation for further analysis. The invention also provides in part devices and methods for improving labeling of nucleic acids including intercalation of nucleic acids using for example mono intercalators such as mono cyanine intercalators. The invention also provides in part methods for analyzing populations nucleic acids including nucleic acids from different subjects or different samples such as bacteria in biomes, and for identifying nucleic acids present in such populations. A non-limiting example is an analysis of bacteria in biomes. Another example is an analysis of DNA from different human subjects.