发明名称 |
Fluorophore-based oligonucleotide probes with a universal element |
摘要 |
A method of detecting the presence of a target polynucleotide and/or sequence variations within the target polynucleotide using a probe system comprising two independent partner oligonucleotide components wherein the first oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence that is labelled with at least one visually detectable label and is not capable of hybridising to the nucleotide sequence of the target polynucleotide; and wherein the second section comprises a nucleotide sequence that is capable of hybridising to a portion of the sequence of the target polynucleotide said second section having a Tm of between 25 and 50oC; and the second oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence which is capable of hybridising to a nucleotide sequence of the first section of the first oligonucleotide; and the second section comprises a nucleotide sequence that is capable of hybridising to a nucleotide sequence of the target polynucleotide that is adjacent to the nucleotide sequence that the second section of the first oligonucleotide is capable of hybridising to; said second section of the second oligonucleotide having a Tm of between 40 and 70oC. There are also provided alternative methods using additional oligonucleotides and probes for use in such methods. |
申请公布号 |
GB2520373(A) |
申请公布日期 |
2015.05.20 |
申请号 |
GB20140010882 |
申请日期 |
2014.06.18 |
申请人 |
LGC LIMITED;UNIVERSITY OF SOUTHAMPTON |
发明人 |
DAVID FRENCH;PAUL GERALD DEBENHAM;REBECCA HOWARD;TOM BROWN;JAMES RICHARDSON |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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