发明名称 |
CONVERSION OF ALPHA-HYDROXYALKYLATED RESIDUES IN BIOMOLECULES USING METHYLTRANSFERASES |
摘要 |
The present invention relates to targeted conversion of alpha-hydroxyalkylated residues in biomolecules in the presence of a directing methyltransferase, namely to targeted removal of the alpha-hydroxyalkyl moieties to give unmodified residues, or targeted derivatization of the alpha-hydroxyalkyl groups by covalent coupling of non-cofactor compounds represented by formula HQ-LX, wherein X represents a functional group or a reporter group attached via a linker moiety L, and QH is selected from HS—, HSe—, HO—H2N—, HN3 or HCN in the presence of a directing methyltransferase. Further development of the method of targeted conversion comprises methods for targeted labeling a biomolecule and method for detecting hydroxymethylated target sites in a biomolecule according to the present invention. |
申请公布号 |
EP2414527(B1) |
申请公布日期 |
2015.05.20 |
申请号 |
EP20100712086 |
申请日期 |
2010.04.01 |
申请人 |
BIOTECHNOLOGIJOS INSTITUTAS |
发明人 |
KLIMASAUSKAS, SAULIUS;LIUTKEVICIUTE, ZITA;KRIUKIENE, EDITA |
分类号 |
C12P7/02;C12P19/28;C12Q1/48 |
主分类号 |
C12P7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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