发明名称 |
FAST PCR FOR STR GENOTYPING |
摘要 |
<p>Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.</p> |
申请公布号 |
EP2539472(B1) |
申请公布日期 |
2015.05.20 |
申请号 |
EP20110748195 |
申请日期 |
2011.02.25 |
申请人 |
LIFE TECHNOLOGIES CORPORATION;HENNESSY, LORI |
发明人 |
WANG, DENNIS |
分类号 |
C12Q1/68;C12N15/10;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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