发明名称 |
METHOD OF IMMOBILISATION OF CHYMOTRYPSIN ON NANOPARTICLES OF SELENIUM OR SILVER |
摘要 |
FIELD: biotechnology.SUBSTANCE: method of immobilisation of chymotrypsin on nanoparticles of selenium or silver is proposed. The solution of chymotrypsin at a concentration of from 1·10to 1 wt % is added the solution of selenious acid in the concentration range of 1.3·10-1.5 wt % or silver nitrate in the concentration range of 1·10-1 wt %. Then ascorbic acid is added to the reaction medium in the concentration of 1·10-0.7 wt % or solution of sodium borohydride in a concentration of from 0.01 to 0.6 wt %. The solution is stirred and left to complete the reaction. The process is carried out at a temperature of from 0 to 50°C.EFFECT: method enables to obtain a stable enzyme-colloidal complex and maintain in it over 90 percent of chymotrypsin activity in a broad pH range, and to increase the catalytic activity of nanocomplexes in certain pH ranges higher than the activity of native chymotrypsin at optimum point.3 tbl, 3 ex |
申请公布号 |
RU2551317(C2) |
申请公布日期 |
2015.05.20 |
申请号 |
RU20130115859 |
申请日期 |
2013.04.10 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUKI INSTITUT VYSOKOMOLEKULJARNYKH SOEDINENIJROSSIJSKOJ AKADEMII NAUK |
发明人 |
ERSHOV DMITRIJ JUR'EVICH;BOROVIKOVA LJUDMILA NIKOLAEVNA;KIPPER AL'BERT IVANOVICH;PISAREV OLEG ALEKSANDROVICH |
分类号 |
C12N11/14;B82B3/00;C01B19/00;C01G5/00;C12N9/76 |
主分类号 |
C12N11/14 |
代理机构 |
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代理人 |
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地址 |
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