Method for bioremediation of highly aromatic hydrocarbon wastes

摘要

Disclosed herein is a method for biopile-based bioremediation of hydrocarbon waste with high aromatic content. The method comprises of isolating specific microorganisms and preparing microbial blend, preparing biopile, adding the microbial blend into the biopile, providing nutrient, aeration and watering of biopile.

主权项

1. A method for bioremediation of highly aromatic hydrocarbon waste, comprising:
a) preparing a microbial blend capable of degrading aromatic hydrocarbons wherein microorganisms in the microbial blend are adsorbed on a biodegradable carrier; b) preparing a biopile comprising a mixture of the hydrocarbon waste, soil and a bulking agent, wherein the highly aromatic hydrocarbon waste has an aromatics content of 100% w/w; c) adding the microbial blend into the biopile followed with addition of nutrients to promote bioremediation reaction; and d) maintaining aeration and moisture of the biopile for a time sufficient to complete the bioremediation of the highly aromatic hydrocarbon waste, wherein the bacteria of the microbial blend are Pseudomonas putida IOCa1 (MTCC No. 5385), Pseudomonas putida IOCR1 (MTCC No. 5802) and Pseudomonas fulva IOCSAM1 (MTCC No. 5387).