Bio-engineered multi-enzyme complexes comprising xylanases and uses thereof

摘要

The present invention relates to bio-engineered multi-enzyme complexes having synergistic enzyme activity comprising xylanases and optionally further comprising additional carbohydrate active enzymes. Such complexes are advantageous for degrading recalcitrant cellulosic biomass.

主权项

1. A bio-engineered multi-enzyme complex comprising:
(i) a scaffold polypeptide comprising a plurality of cohesin domains of divergent specificities and at least one substrate-binding module; and (ii) a plurality of carbohydrate active enzymes bound to said scaffold polypeptide, each carbohydrate active enzyme comprises a divergent dockerin domain matching one of the plurality of cohesin domains of the scaffold polypeptide;wherein the plurality of carbohydrate active enzymes comprise at least two different xylanases, wherein at least one of the two different xylanases is a non-cellulosomal xylanase bio-engineered with a heterologous dockerin domain.