Metabolic maturation in stem cell-derived tissue cells

摘要

The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.

主权项

1. A method of maturing human induced pluripotent cell-derived (human iPS-derived) cells, comprising obtaining immature, human differentiated cells that have been prepared in vitro from human iPS cells and exposing the immature, human differentiated iPS cell-derived cells to a defined media that is essentially glucose-free, essentially serum-free, and which comprises one or more fatty acids, to mature the immature, human differentiated iPS cell-derived cells to a more mature state.