COMPUTATIONALLY OPTIMIZED BROADLY REACTIVE ANTIGENS FOR H3N2 H2N2 AND B INFLUENZA VIRUSES

摘要

Described herein is the generation of optimized H3N2 H2N2 and B influenza HA polypeptides for eliciting a broadly reactive immune response to influenza virus isolates. The optimized HA polypeptides were developed through a series of HA protein alignments and subsequent generation of consensus sequences based on H3N2 H2N2 and B influenza isolates. Provided herein are optimized H3N2 H2N2 and B influenza HA polypeptides and compositions fusion proteins and VLPs comprising the HA polypeptides. Further provided are codon optimized nucleic acid sequences encoding the HA polypeptides. Methods of eliciting an immune response against influenza virus in a subject are also provided by the present disclosure.