ELECTRON MICROSCOPIC EXAMINATION METHOD FOR EXAMINING BIOSAMPLE WHILE KEEPING SAID BIOSAMPLE UNCHANGED AND COMPOSITION FOR EVAPORATION INHIBITION UNDER VACUUM SCANNING ELECTRON MICROSCOPE AND TRANSMISSION ELECTRON MICROSCOPE FOR USE IN SAID METHOD

摘要

A method for electron microscopic examination is provided with which it is possible to examine a biosample with an electron microscope while keeping the biosample alive and to observe the biosample being moving. Also provided are a composition for evaporation inhibition under vacuum a scanning electron microscope and a transmission electron microscope which are for use in the method. This method for sample examination by electron microscopy is characterized by comprising: a step in which a composition for evaporation inhibition that comprises at least one ingredient selected from amphipathic compounds oils and fats and ionic liquids is applied to the surface of a sample to form a thin film and thus cover the sample with the thin film; and a step in which an electron microscopic image of the thin film covered sample that has been placed in a vacuum sample chamber is displayed in a display device.