发明名称 MRM-MS SIGNATURE ASSAY
摘要 The present invention relates to mass spectrometry methods employing multiple reaction monitoring (MRM) in the field of cancer therapeutics, specifically prostate cancer and melanoma.
申请公布号 US2015133342(A1) 申请公布日期 2015.05.14
申请号 US201314398794 申请日期 2013.05.03
申请人 Nuclea Biotechnologies, Inc. 发明人 Tam Sun W.;Xu Ying;Muraca Patrick J.
分类号 G01N33/68;C12Q1/37 主分类号 G01N33/68
代理机构 代理人
主权项 1. A method of determining the concentration of a prostate cancer biomarker or affiliated molecular partner in a sample comprising: (a) obtaining a sample from a subject; (b) treating said sample to digest one or more prostate cancer biomarkers or affiliated molecular partners contained therein; (c) generating a mass spectrometry profile of the digested sample of step (b); (d) comparing the mass spectrometry profile from step (c) to a standard curve, wherein said standard curve has been created using at least one calibration standard selected from the group consisting of SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO 28, SEQ ID NO. 29 SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43 and combinations thereof; and (e) calculating a concentration of said one or more prostate cancer biomarkers or affiliated molecular partners in the sample obtained from the subject based on the standard curve.
地址 Pittsfield MA US