METHODS OF DETECTING CELLS LATENTLY INFECTED WITH HIV

摘要

Disclosed are methods of detecting and isolating CD4+ T cells latently infected with HIV as well as methods of screening for inhibitors of CD4+ T cells latently infected with HIV.

主权项

1. A method of identifying a latently infected CD4+ T cell from a subject, the method comprising:
obtaining a biological sample from the subject, the biological sample comprising CD4+ T cells; contacting the sample with a first labeled antibody comprising a first label, wherein the first labeled antibody specifically binds PD-1, LAG-3, or TIGIT; forming a complex between the first labeled antibody to PD-1, LAG-3, or TIGIT with PD-1, LAG-3, or TIGIT; detecting the complex by detecting the first label on the surface of a CD4+ cell that has bound the labeled antibody in the complex; wherein detection of the first label indicates that the cell is a latently infected CD4+ T cell.