Method for Molecular Authentication of Panax ginseng and Panax quinquefolius Using SNP Markers in Dammarenediol Synthase (DS) Gene

摘要

A method for discriminating Panax ginseng, P. quinquefolius and related products and commodities thereof using single nucleotide polymorphism (SNP) is provided, thereby discriminating the P ginseng and P. quinquefolius rapidly and easily using a small amount of ginseng samples. CONSTITUTION: A method for discriminating P ginseng and P. quinquefolius and related products comprises the steps of: extracting DNA from the P. ginseng and P quinquefolius samples; PCR amplifying of dammarenediol synthase (DS) gene region performing on the extracted DNA using a pair of DS primers; comparing the DS gene fragment of the P ginseng and P quinquefolius to prepare two specific SNP primers for P ginseng and P quinquefolius respectively; amplifying the PCR products of DS region of the P ginseng and P quinquefolius using the specific SNP primers by nested-PCR, subjecting the amplified DNA to electrophoresis, and comparing the electrophoresis band patterns. P1 5' P3 5' 5' X3 Exon Unknow Intron Nl Obtained Intron NQ Forward primer Reverse primer IvO FRK Reaction PCRP5 571 bp amplification 341 bp JN/596111 Amplified Sequences