METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIAL CELLS

摘要

The present invention provides a method for producing retinal pigment epithelial cells, the method including: (1) a first step for forming an aggregate of pluripotent stem cells by suspension-culturing pluripotent stem cells in a serum-free medium; (2) a second step for suspension-culturing the aggregate formed in step (1) in a serum-free medium or serum medium including a BMP signal transduction pathway agonist and not including a sonic hedgehog signal transduction pathway agonist, and obtaining an aggregate including retinal precursor cells; and (3) a third step for suspension-culturing the aggregate obtained in step (2) in a serum-free medium or serum medium including a Wnt signal pathway agonist and not including a sonic hedgehog signal transduction pathway agonist or a BMP signal transduction pathway agonist, and obtaining an aggregate including retinal pigment epithelial cells.