| 主权项 |
1. A method of purifying at least one procyanidin oligomer DP3 to DP12 from a cocoa-containing or cocoa-derived sample by liquid-liquid partition chromatography, the method comprising
defatting the cocoa sample; selecting a solvent system of a mobile phase lipophillic solvent, a bridging solvent of one or more alcohols (C1 to C4), and water or aqueous solvent, wherein the stationary phase can be selected as either the lipophilic solvent or water or aqueous solvent, and the mobile phase is selected as the other; mixing the cocoa sample with the mobile and stationary phase solvents; filling with stationary phase a rotor of a CPC having multiple interconnected mixing chambers within it, each mixing chamber containing the stationary phase solvent, and allowing the mixture of the cocoa-containing sample to be introduced into the rotor mixing chambers at one end; spinning the rotor to allow the cocoa-containing sample to flow through multiple mixing chambers with the mobile phase solvent; and collecting the at least one procyanidin oligomer DP3 to DP12. |
